Reusable Microfluidic Chambers for Single-Molecule Microscopy.

ABSTRACT: Maintaining a consistent environment in single-molecule microfluidic chambers containing surface-bound molecules requires laborious cleaning and surface passivation procedures. Despite such efforts, variations in nonspecific binding and background signals commonly occur across different chambers. Being able to reuse the chambers without degrading the surface promises significant practical and fundamental advantages; however, this necessitates removing the molecules attached to the surface, such as DNA, proteins, lipids, or nanoparticles. Biotin-streptavidin attachment is widely used for such attachments, as biotin can be readily incorporated into these molecules. In this study, we present single-molecule fluorescence experiments that demonstrate effective resetting and recycling of the cha

SUBMITTER: Alfehaid J 

PROVIDER: S-EPMC11505897 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature