Mycotoxin Detection through Colorimetric Immunoprobing with Gold Nanoparticle Antibody Conjugates.

ABSTRACT: Driven by their exceptional optical characteristics, robust chemical stability, and facile bioconjugation, gold nanoparticles (AuNPs) have emerged as a preferred material for detection and biosensing applications in scientific research. This study involves the development of a simple, rapid, and cost-effective colorimetric immuno-sensing probe to detect aflatoxin B1 and zearalenone using AuNP antibody (AuNP-mAb) conjugates. Anti-toxin antibodies were attached to the AuNPs by using the physical adsorption method. The colorimetric immunosensor developed operates on the principle that the optical properties of the AuNP are very sensitive to aggregation, which can be induced by a critical high salt concentration. Although the presence of antibodies on the AuNP surface inhibits the aggregation,

SUBMITTER: Sharma V 

PROVIDER: S-EPMC11506043 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature