Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication.

ABSTRACT: Biomolecular assemblies are fundamental to life and viral disease. The spatiotemporal coordination of viral replication and assembly is largely unknown. Here, we developed a dual-color click chemistry procedure for imaging adenovirus DNA (vDNA) replication in the cell nucleus. Late- but not early-replicated vDNA was packaged into virions. Early-replicated vDNA segregated from the viral replication compartment (VRC). Single object tracking, superresolution microscopy, fluorescence recovery after photobleaching, and correlative light-electron microscopy revealed a stepwise assembly program involving vDNA and capsid intermediates. Depending on replication and the scaffolding protein 52K, late-replicated vDNA with rapidly exchanging green fluorescent protein-tagged capsid linchpin protein V an

SUBMITTER: Gomez-Gonzalez A 

PROVIDER: S-EPMC11506174 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature