Cap-Specific m⁶Am Methyltransferase PCIF1/CAPAM Regulates mRNA Stability of <i>RAB23</i> and <i>CNOT6</i> through the m⁶A Methyltransferase Activity.

ABSTRACT: Chemical modifications of cellular RNAs play key roles in gene expression and host defense. The cap-adjacent N⁶,2'-O-dimethyladenosine (m⁶Am) is a prevalent modification of vertebrate and viral mRNAs and is catalyzed by the newly discovered N⁶ methyltransferase PCIF1. However, its role in gene expression remains unclear due to conflicting reports on its effects on mRNA stability and translation. In this study, we investigated the impact of siRNA-mediated transient suppression of PCIF1 on global mRNA expression in HeLa cells. We identified a subset of differentially expressed genes (DEGs) that exhibited minimal overlap with previously reported DEGs. Subsequent validation revealed that PCIF1 positively and negatively regulates RAB23 and C

SUBMITTER: Sugita A 

PROVIDER: S-EPMC11506431 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature