Ultrasensitive Electrochemical Detection of <i>Salmonella typhimurium</i> in Food Matrices Using Surface-Modified Bacterial Cellulose with Immobilized Phage Particles.

ABSTRACT: The rapid and sensitive detection of Salmonella typhimurium in food matrices is crucial for ensuring food safety. This study presents the development of an ultrasensitive electrochemical biosensor using surface-modified bacterial cellulose (BC) integrated with polypyrrole (Ppy) and reduced graphene oxide (RGO), further functionalized with immobilized S. typhimurium-specific phage particles. The BC substrate, with its ultra-fibrous and porous structure, was modified through in situ oxidative polymerization of Ppy and RGO, resulting in a highly conductive and flexible biointerface. The immobilization of phages onto this composite was facilitated by electrostatic interactions between the polycationic Ppy and the negatively charged phage capsid heads, optimizing phage orientation

SUBMITTER: Hussain W 

PROVIDER: S-EPMC11506579 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature