Refinement and Enhancement of <i>Agrobacterium</i>-Mediated Transient Transformation for Functional Gene Examination in Mulberry (<i>Morus</i> L.).

ABSTRACT: Background: Mulberry (Morus L.), a vital perennial woody plant with significant economic importance, is utilized for silkworm rearing, human consumption and medicinal use. The availability of mulberry's whole-genome sequencing data has underscored the demand for an effective, user-friendly, and high-throughput protocol to facilitate the elucidation of gene functions. Methods and Results: In this investigation, we established a transient transformation approach using Agrobacterium tumefaciens-mediated sonication followed by vacuum infiltration in mulberry tissue culture seedlings. Simultaneously, we optimized the transformation conditions, including mulberry genotypes, A. tumefaciens strain, acetosyringone concentration, bacterial density, sonication time,

SUBMITTER: Mo R 

PROVIDER: S-EPMC11507023 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature