Selection of Reference Genes and HSP17.9A Expression Profiling in Heat-Stressed Grapevine Varieties.

ABSTRACT:

Background

"Touriga Franca" (TF) and "Touriga Nacional" (TN) are grapevine varieties cultivated in the 'Douro Superior' subregion (Northern Portugal) that experience stressful environmental conditions during the summer.

Objectives

Aiming to profile the expression of stress-responsive genes by quantitative real-time PCR (qPCR) in TF and TN plants growing naturally, three candidate reference genes were first tested under controlled conditions.

Methods

To simulate a summer's day, TF and TN in vitro plants were exposed to 32 °C-3 h (heat acclimation) and 42 °C-1 h (severe heat stress, HS) followed by two recovery periods (32 °C-3 h and 24 °C-24 h). Leaf samples were collected at the end of each phase. Control plants were kept at 24 °C.

Results

Among the candidate r

SUBMITTER: Carvalho A 

PROVIDER: S-EPMC11507026 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature