Diversity, Distribution and Structural Prediction of the Pathogenic Bacterial Effectors EspN and EspS.

ABSTRACT:

Background

Many Gram-negative enterobacteria translocate virulence proteins (effectors) into intestinal epithelial cells using a type III secretion system (T3SS) to subvert the activity of various cell functions possess. Many T3SS effectors have been extensively characterized, but there are still some effector proteins whose functional information is completely unknown.

Methods

In this study, two predicted effectors of unknown function, EspN and EspS (Escherichia coli secreted protein N and S), were selected for analysis of translocation, distribution and structure prediction.

Results

The TEM1 (β-lactamase) translocation assay was performed, which showed that EspN and EspS are translocated into host cells in a T3SS-dependent manner during bacterial infection. A

SUBMITTER: Li Z 

PROVIDER: S-EPMC11507257 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature