Next-Generation Sequencing to Determine Changes in the Intestinal Microbiome of Juvenile Sturgeon Hybrid (<i>Acipenser gueldenstaedtii</i>♀ × <i>Acipenser baerii</i>♂) Resulting from Sodium Butyrate, Β-Glucan and Vitamin Supplementation.

ABSTRACT:

Background/objectives

The effect of sodium butyrate (NaB), β-glucan (βG) and vitamins in the diet on gut microbiome, cortisol level, lysozyme activity and growth parameters of juvenile hybrid sturgeon (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) was determined.

Methods

Sturgeon hybrids (n = 144) were divided into three groups with enriched feeding (mg/kg of feed): FQV1 (50 NaB; 20 βG; const. vitamins), FQV2 (150 NaB; 20 βG; const. vitamins), FQV3 (50 NaB; 60 βG; const. vitamins) and control (not supplemented), each group in triplicate, 12 fish in each repetition. Rearing was carried out for 30 days in controlled conditions. Gut microbiome was characterized using Next Generation Sequencing (NGS) of DNA samples isolated from intestinal content. Cortiso

SUBMITTER: Arciuch-Rutkowska M 

PROVIDER: S-EPMC11507316 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature