Integrated Transcriptomics and Metabolomics Reveal Key Insights into Iridoid Biosynthesis in <i>Gentiana crassicaulis</i> Seeds during Germination.

ABSTRACT: Background:Gentiana crassicaulis Duthie ex Burk., a key species used in traditional Chinese medicine for treating rheumatic pain and stroke, contains iridoids as its primary active component. However, the biosynthetic mechanisms underlying iridoid production are not fully understood. Methods: This study focused on iridoid biosynthesis during the germination of G. crassicaulis seeds, integrating metabolomic and transcriptomic analyses to uncover the underlying pathways and key candidate genes. Results: 196,132 unigenes and 10 iridoid compounds were identified through RNA-seq and ultra performance liquid chromatography-quadrupole time of flight-mass spectrometer (UPLC-Q-TOF-MS), respectively. The intersection of results from Pearson correlation analysis and

SUBMITTER: Xuan L 

PROVIDER: S-EPMC11507440 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature