Disrupted Post-Transcriptional Regulation of Gene Expression as a Hallmark of Fatty Liver Progression.

ABSTRACT: It is known that both transcriptional and post-transcriptional mechanisms control messenger RNA (mRNA) levels. Compared to transcriptional regulations, our understanding of how post-transcriptional regulations adapt during fatty liver progression at the whole-transcriptome level is unclear. While traditional RNA-seq analysis uses only reads mapped to exons to determine gene expression, recent studies support the idea that intron-mapped reads can be reliably used to estimate gene transcription. In this study, we analyzed differential gene expression at both the exon and intron levels using two liver RNA-seq datasets from mice that were fed a high-fat diet for seven weeks (mild fatty liver) or thirty weeks (severe fatty liver). We found that the correlation between gene transcription and mat

SUBMITTER: Takaoka S 

PROVIDER: S-EPMC11507451 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature