HybridQC: A SNP-Based Quality Control Application for Rapid Hybridity Verification in Diploid Plants.

ABSTRACT: Background/Objectives: Hybridity authentication is an important component of quality assurance and control (QA/QC) in breeding programs. Here, we introduce HybridQC v1.0, a QA/QC software program specially designed for parental purity and hybridity determination. HybridQC rapidly detects molecular marker polymorphism between parents of a cross and utilizes only the informative markers for hybridity authentication. Methods: HybridQC is written in Python and designed with a graphical user interface (GUI) compatible with Windows operating systems. We demonstrated the QA/QC analysis workflow and functionality of HybridQC using Kompetitive allele-specific PCR (KASP) SNP genotype data for cowpea (Vigna unguiculata). Its performance was validated in other crop data, including

SUBMITTER: Ongom PO 

PROVIDER: S-EPMC11507623 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature