A Streamlined High-Throughput LC-MS Assay for Quantifying Peptide Degradation in Cell Culture.

ABSTRACT: Peptides are widely used in biomaterials due to their easy of synthesis, ability to signal cells, and modify the properties of biomaterials. A key benefit of using peptides is that they are natural substrates for cell-secreted enzymes, which creates the possibility of utilizing cell-secreted enzymes for tuning cell-material interactions. However, these enzymes can also induce unwanted degradation of bioactive peptides in biomaterials, or in peptide therapies. Liquid chromatography-mass spectrometry (LC-MS) is a widely used, powerful methodology that can separate complex mixtures of molecules and quantify numerous analytes within a single run. There are several challenges in using LC-MS for the multiplexed quantification of cell-induced peptide degradation, including the need for non-degrad

SUBMITTER: Rozans SJ 

PROVIDER: S-EPMC11507709 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature