Hu.MAP3.0: Atlas of human protein complexes by integration of > 25,000 proteomic experiments.

ABSTRACT: Macromolecular protein complexes carry out most functions in the cell including essential functions required for cell survival. Unfortunately, we lack the subunit composition for all human protein complexes. To address this gap we integrated >25,000 mass spectrometry experiments using a machine learning approach to identify > 15,000 human protein complexes. We show our map of protein complexes is highly accurate and more comprehensive than previous maps, placing ~75% of human proteins into their physical contexts. We globally characterize our complexes using protein co-variation data (ProteomeHD.2) and identify co-varying complexes suggesting common functional associations. Our map also generates testable functional hypotheses for 472 uncharacterized proteins which we support using AlphaFo

SUBMITTER: Fischer SN 

PROVIDER: S-EPMC11507723 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature