Torsion is a Dynamic Regulator of DNA Replication Stalling and Reactivation.

ABSTRACT: The inherent helical structure of DNA dictates that a replisome must rotate relative to DNA during replication, presenting inevitable topological challenges to replication. However, little is known about how the replisome progresses against torsional stress. Here, we developed a label-free, high-resolution, real-time assay to monitor replisome movement under torsion. We visualized the replisome rotation of DNA and determined how the replisome slows down under torsion. We found that while helicase or DNA polymerase (DNAP) individually is a weak torsional motor, the replisome composed of both enzymes is the most powerful DNA torsional motor studied to date. It generates ~ 22 pN·nm of torque before stalling, twice the stall torque of E. coli RNA polymerase. Upon replisome stalling, the

SUBMITTER: Jia X 

PROVIDER: S-EPMC11507786 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature