Multiplex Determination of K-Antigen and Colanic Acid Capsule Variants of <i>Cronobacter sakazakii</i>.

ABSTRACT: Cronobacter sakazakii is associated with the ingestion of contaminated reconstituted powdered infant formula (PIF), resulting in necrotizing enterocolitis, sepsis and meningitis in neonatal infants. Potential virulence determinants include the variable capsular polysaccharides; K-antigen and colanic acid (CA). Strains encoding for the capsule variant K2:CA2 have been strongly associated with neonatal meningitis cases. This study aimed to develop and apply a multiplex PCR assay to determine C. sakazakii K-antigen and colanic acid types. Twenty-six strains of C. sakazakii which had previously been isolated from food and environmental sources were used. These cover 18 multilocus sequence types and four serotypes. Based on our research findings, we have identified two K-an

SUBMITTER: Ibrahim KM 

PROVIDER: S-EPMC11507822 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature