YebC2 resolves ribosome stalling at polyprolines independent of EF-P and the ABCF ATPase YfmR.

ABSTRACT: Polyproline motifs are essential structural features of many proteins, and recent evidence suggests that EF-P is one of several factors that facilitate their translation. For example, YfmR was recently identified as a protein that prevents ribosome stalling at proline-containing sequences in the absence of EF-P. Here, we show that the YebC-family protein YebC2 (formerly YeeI) functions as a translation factor in B. subtilis that resolves ribosome stalling at polyprolines. We demonstrate that YebC2, EF-P and YfmR act independently to support cellular fitness. Moreover, we show that YebC2 interacts directly with the 70S ribosome, supporting a direct role for YebC2 in translation. Finally, we assess the evolutionary relationship between YebC2 and other characterized YebC family protein

SUBMITTER: Hong HR 

PROVIDER: S-EPMC11507958 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature