Determination of the mean duration of recent infection and false recency rate for the HIV triplex multiplex bead assay.

ABSTRACT:

Background

We developed the HIV Triplex multiplex bead assay to identify and serotype HIV infection with high sensitivity and specificity; and distinguish recent from long-term HIV-1 infections. It can facilitate accurate incidence estimation, while reducing the number of tests and blood collected, which is highly desirable for use in future studies and surveys. Using previously collected, treatment-naive longitudinal seroconversion HIV-1 positive panels and specimens from individuals infected for >12 months, we determined the assay's mean duration of recent infection (MDRI) and false-recency rate (FRR) respectively, at various mean fluorescent intensity (MFI) cutoffs.

Methods

We tested seroconversion specimens (N = 814) from 142 individuals infected with HIV-1 subtypes B, C

SUBMITTER: Domaoal RA 

PROVIDER: S-EPMC11508083 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature