Light Regulated CoWRKY15 Acts on CoSQS Promoter to Promote Squalene Synthesis in Camellia oleifera Seeds.

ABSTRACT: Squalene synthase (SQS) is the most direct key enzyme regulating squalene synthesis. To better understand the regulatory mechanisms of squalene biosynthesis, a 1423-bp long promoter region of the CoSQS gene was isolated from Camellia oleifera. Plant CARE and PLACE analysis affirmed the existence of the core promoter elements such as TATA and CAAT boxes and transcription factor binding sites like W-box and MYB in the isolated sequence. Exogenous factors regulating the CoSQS promoter were obtained by using Yeast one-hybrid screening, and the key transcription factor CoWRKY15 was found. AOS (Antibody Optimization System) analysis showed that CoWRKY15 had the highest interactions with a confidence level of 0.9026. Bioinformatics analysis showed that CoWRKY15 belonged to cl

SUBMITTER: Li A 

PROVIDER: S-EPMC11508267 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature