Proteomic Analysis of Human Serum Proteins Adsorbed onto Collagen Barrier Membranes.

ABSTRACT: Collagen barrier membranes are frequently used in guided tissue and bone regeneration. The aim of this study was to analyze the signature of human serum proteins adsorbed onto collagen membranes using a novel protein extraction method combined with mass spectrometry. Native porcine-derived collagen membranes (Geistlich Bio-Gide^®, Wolhusen, Switzerland) were exposed to pooled human serum in vitro and, after thorough washing, subjected to protein extraction either in conjunction with protein enrichment or via a conventional surfactant-based method. The extracted proteins were analyzed via liquid chromatography with tandem mass spectrometry. Bioinformatic analysis of global profiling, gene ontology, and functional enrichment of the identified proteins was performed. Overall, a tota

SUBMITTER: Shanbhag S 

PROVIDER: S-EPMC11508515 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature