A Simple Model to Study Mosaic Gene Expression in 3D Endothelial Spheroids.

ABSTRACT:

Aims

The goal of this study was to establish a simple model of 3D endothelial spheroids with mosaic gene expression using adeno-associated virus (AAV) transduction, with a future aim being to study the activity of post-zygotic mutations common to vascular malformations.

Methods

In this study, 96-well U-bottom plates coated with a commercial repellent were seeded with two immortalized human endothelial cell lines and aggregation monitored using standard microscopy or live-cell analysis. The eGFP expression was used to monitor the AAV transduction.

Results

HUVEC-TERT2 could not form spheroids spontaneously. The inclusion of collagen I in the growth medium could stimulate cell aggregation; however, these spheroids were not stable. In contrast, the hCMEC/D3 cells aggregat

SUBMITTER: McRobb LS 

PROVIDER: S-EPMC11508842 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature