Single nucleotide extension technology for quantitative site-specific evaluation of metC/C in GC-rich regions.

ABSTRACT: The development and use of high throughput technologies for detailed mapping of methylated cytosines (metC) is becoming of increasing importance for the expanding field of epigenetics. The single nucleotide primer extension reaction used for genotyping of single nucleotide polymorphisms has been recently adapted to interrogate the bisulfite modification induced 'quantitative' C/T polymorphism that corresponds to metC/C in the native DNA. In this study, we explored the opportunity to investigate C/T (and G/A) ratios using the Applied Biosystems (ABI) SNaPshot technology. The main effort of this study was dedicated to addressing the complexities in the analysis of DNA methylation in GC-rich regions where interrogation of the target cytosine can be confounded by variable degrees of methylatio

SUBMITTER: Kaminsky ZA 

PROVIDER: S-EPMC1150895 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature