Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor.

ABSTRACT: Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5-20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R² values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-

SUBMITTER: Zea M 

PROVIDER: S-EPMC11509315 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature