Novel <i>Agrobacterium fabrum</i> str. 1D1416 for Citrus Transformation.

ABSTRACT: Citrus is one of the world's most important and widely produced fruit crops, with over a 100 million metric tons harvested from nearly 10 million hectares in 2023. Challenges in crop maintenance, production, and fruit quality necessitate developing new traits through Agrobacterium-mediated genetic transformation. While a few Agrobacterium strains (EHA105, GV3101, LBA4404) are known to transform citrus, many wild strains remain untested. We screened forty-one wild-type Agrobacterium strains isolated from various woody species and identified five capable of DNA transfer into citrus cells. Strain 1D1416 demonstrated the highest transient transformation frequency in Carrizo epicotyl explants (88%), outperforming the control EHA105 (84%) with comparable shoot regeneration rates (3

SUBMITTER: Alabed D 

PROVIDER: S-EPMC11509345 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature