Detection of Nitrate-Reducing/Denitrifying Bacteria from Contaminated and Uncontaminated Tallgrass Prairie Soil: Limitations of PCR Primers.

ABSTRACT: Contamination of soil by spills of crude oil and oilfield brine is known to affect the species composition and functioning of soil microbial communities. However, the effect of such contamination on nitrogen cycling, an important biogeochemical cycle in tallgrass prairie soil, is less well known. Detecting nitrate-reducing (NR) and denitrifying (DN) bacteria via PCR amplification of the genes essential for these processes depends on how well PCR primers match the sequences of these bacteria. In this study, we enriched for NR and DN bacteria from oil/brine tallgrass prairie soil contaminated 5-10 years previously versus those cultured from uncontaminated soil, confirmed the capacity of 75 strains isolated from the enrichments to reduce nitrate and/nitrite, then screened the strains with pri

SUBMITTER: AbuBakr SM 

PROVIDER: S-EPMC11509419 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature