Multienzyme Immobilization on PVDF Membrane via One-Step Mussel-Inspired Method: Enhancing Fouling Resistance and Self-Cleaning Efficiency.

ABSTRACT: Immobilizing different enzymes on membranes can result in biocatalytic active membranes with a self-cleaning capacity toward a complex mixture of foulants. The membrane modification can reduce fouling and enhance filtration performance. Protease, lipase, and amylase were immobilized on poly(vinylidene fluoride) (PVDF) microfiltration membranes using a polydopamine coating in a one-step method. The concentrations of polydopamine precursor and enzymes were optimized during the immobilization. The higher hydrolytic activities were obtained using 0.2 mg/mL of dopamine hydrochloride and 4 mg/mL of enzymes: 0.90 mg_starch/min·cm² for amylase, 10.16 nmol_tyrosine/min·cm² for protease, and 20.48 µmol_{p-nitrophenol}/min·cm² for lipase. F

SUBMITTER: Mulinari J 

PROVIDER: S-EPMC11509426 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature