Analysis of the Respiratory Activity in the Antarctic Yeast <i>Rhodotorula mucilaginosa</i> M94C9 Reveals the Presence of Respiratory Supercomplexes and Alternative Elements.

ABSTRACT: The respiratory activities of mitochondrial complexes I, II, and IV were analyzed in permeabilized Rhodotorula mucilaginosa cells and isolated mitochondria, and the kinetic parameters K_0.5 and V_max were obtained. No difference in substrate affinities were found between mitochondria and permeabilized cells. The activities of the components of the mitochondrial respiratory chain of the Antarctic yeast R. mucilaginosa M94C9 were identified by in-gel activity and SDS-PAGE. The mitochondria exhibited activity for the classical components of the electron transport chain (Complexes I, II, III, and IV), and supercomplexes were formed by a combination of the respiratory complexes I, III, and IV. Unfortunately, the activities of the monomeric and dimeric forms of

SUBMITTER: Reyes-Rosario D 

PROVIDER: S-EPMC11509550 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature