Limited column formation in the embryonic growth plate implies divergent growth mechanisms during pre- and postnatal bone development.

ABSTRACT: Chondrocyte columns, which are a hallmark of growth plate architecture, play a central role in bone elongation. Columns are formed by clonal expansion following rotation of the division plane, resulting in a stack of cells oriented parallel to the growth direction. In this work, we analyzed hundreds of Confetti multicolor clones in growth plates of mouse embryos using a pipeline comprising 3D imaging and algorithms for morphometric analysis. Surprisingly, analysis of the elevation angles between neighboring pairs of cells revealed that most cells did not display the typical stacking pattern associated with column formation, implying incomplete rotation of the division plane. Morphological analysis revealed that although embryonic clones were elongated, they formed clusters oriented perpend

SUBMITTER: Rubin S 

PROVIDER: S-EPMC11509684 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature