Genomic and Transcriptomic Analyses Identify Two Key Glycosyltransferase Genes <i>alhH</i> and <i>alhK</i> of Exopolysaccharide Biosynthesis in <i>Pantoea alhagi</i> NX-11.

ABSTRACT: The exopolysaccharide (EPS) produced by Pantoea alhagi NX-11, referred to as alhagan, enhances plant stress resistance, improves soil properties, and exhibits notable rheological properties. Despite these benefits, the exact bio-synthetic process of alhagan by P. alhagi NX-11 remains unclear. This study focused on sequencing the complete genome of P. alhagi NX-11 and identifying an alhagan synthesis gene cluster (LQ939_RS12550 to LQ939_RS12700). Gene annotation revealed that alhagan biosynthesis in P. alhagi NX-11 follows the Wzx/Wzy-dependent pathway. Furthermore, transcriptome analysis of P. alhagi NX-11 highlighted significant upregulation of four glycosyltransferase genes (alhH, wcaJ, alhK, and alhM) within th

SUBMITTER: He K 

PROVIDER: S-EPMC11509785 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature