Effects of Inosine-5'-monophosphate Dehydrogenase (IMPDH/GuaB) Inhibitors on <i>Borrelia burgdorferi</i> Growth in Standard and Modified Culture Conditions.

ABSTRACT: Borrelia burgdorferi's inosine-5'-monophosphate dehydrogenase (IMPDH, GuaB encoded by the guaB gene) is a potential therapeutic target. GuaB is necessary for B. burgdorferi replication in mammalian hosts but not in standard laboratory culture conditions. Therefore, we cannot test novel GuaB inhibitors against B. burgdorferi without utilizing mammalian infection models. This study aimed to evaluate modifications to a standard growth medium that may mimic mammalian conditions and induce the requirement of GuaB usage for replication. The effects of two GuaB inhibitors (mycophenolic acid, 6-chloropurine riboside at 125 μM and 250 μM) were assessed against B. burgdorferi (guaB+) grown in standard Barbour-Stoenner-Kelly-II (BSK-II) medium (6% rabbit seru

SUBMITTER: Siegel EL 

PROVIDER: S-EPMC11509813 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature