High-Sensitivity RT-LAMP for Molecular Detection of O'nyong-nyong (<i>Alphavirus onyong</i>).

ABSTRACT: Mosquitoes serve as vectors for many arthropod-borne viruses (arboviruses) that are responsible for millions of human infections and thousands of deaths each year. Among these arboviruses, O'nyong-nyong virus (ONNV) is an African alphavirus mainly transmitted by Anopheles mosquitoes. ONNV can be detected through serological or molecular tests, the first showing cross-reactivity to co-circulating alphaviruses and requiring technically demanding confirmation, while the latter, usually based on real-time PCR, are costly and demand specific equipment. Isothermal amplification approaches, such as Loop-Mediated Isothermal Amplification (LAMP), should therefore provide a cost-effective, sensitive, and specific alternative for virus detection, suitable for the resource-limited regions where

SUBMITTER: Faisca-Silva D 

PROVIDER: S-EPMC11510077 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature