Reversible Photochromic Reactions of Bacteriorhodopsin from <i>Halobacterium salinarum</i> at Femto- and Picosecond Times.

ABSTRACT: The operation of bacteriorhodopsin (BR) from the archaeon Halobacterium salinarum is based on the photochromic reaction of isomerization of the chromophore group (the retinal protonated Schiff base, RPSB) from the all-trans to the 13-cis form. The ultrafast dynamics of the reverse 13-cis → all-trans photoreaction was studied using femtosecond transient absorption spectroscopy in comparison with the forward photoreaction. The forward photoreaction was initiated by photoexcitation of BR by pulse I (540 nm). The reverse photoreaction was initiated by photoexcitation of the product K₅₉₀ at an early stage of its formation (5 ps) by pulse II (660 nm). The conversion of the excited K₅₉₀ to the ground state procee

SUBMITTER: Smitienko O 

PROVIDER: S-EPMC11510181 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature