Role of the SaeRS Two-Component Regulatory System in Group B <i>Streptococcus</i> Biofilm Formation on Human Fibrinogen.

ABSTRACT: Streptococcus agalactiae, also known as Group B Streptococcus or GBS, is a commensal colonizer of human vaginal and gastrointestinal tracts that can also be a deadly pathogen for newborns, pregnant women, and the elderly. The SaeRS two-component regulatory system (TCS) positively regulates the expression of two GBS adhesins genes, but its role in the formation of biofilm, an important step in pathogenesis, has not been investigated. In the present study, we set up a novel model of GBS biofilm formation using surfaces coated with human fibrinogen (hFg). Biofilm mass and structure were analyzed by crystal violet staining and three-dimensional fluorescence microscopy, respectively. GBS growth on hFg resulted in the formation of a mature and abundant biofilm composed of bacterial

SUBMITTER: Coppolino F 

PROVIDER: S-EPMC11510217 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature