Ultrasound-Assisted Solid-Phase Affibody Synthesis Using Z_EGFR:1907 as an Example-Superior to the Conventional Protocol?

ABSTRACT:

Background

Affibody molecules represent a class of highly specific binders of particular interest for the development of highly affine target-specific radiopharmaceuticals. Their chemical synthesis is, however, intricate due to their considerable length of 58 amino acids; thus, approaches to optimize their preparation are constantly being sought.

Methods

As ultrasound assistance has recently been shown to increase the efficiency of amino acid conjugation during solid-phase peptide synthesis (SPPS), the influence of ultrasonication on the outcome of the SPPS-based preparation of the EGFR-specific affibody Z_EGFR:1907 was compared to a common protocol relying on mechanical shaking.

Results

After the identification of a suitable solid support for the study, the

SUBMITTER: Prochiner M 

PROVIDER: S-EPMC11510254 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature