PA subunit from influenza virus polymerase complex interacts with a cellular protein with homology to a family of transcriptional activators.

ABSTRACT: The PA subunit of the influenza virus polymerase complex is a phosphoprotein that induces proteolytic degradation of coexpressed proteins. Point mutants with reduced proteolysis induction reconstitute viral ribonucleoproteins defective in replication but not in transcriptional activity. To look for cellular factors that could associate with PA protein, we have carried out a yeast two-hybrid screen. Using a human kidney cDNA library, we identified two different interacting clones. One of them was identified as the human homologue of a previously described cDNA clone from Gallus gallus called CLE. The human gene encodes a protein of 36 kDa (hCLE) and is expressed ubiquitously in all human organs tested. The interaction of PA and hCLE was also observed with purified proteins in vitro by using

SUBMITTER: Huarte M 

PROVIDER: S-EPMC115105 | biostudies-literature | 2001 Sep

REPOSITORIES: biostudies-literature