Impact of Protein Coronas on Lipid Nanoparticle Uptake and Endocytic Pathways in Cells.

ABSTRACT: Lipid nanoparticles (LNPs), widely used in disease diagnosis and drug delivery, face the challenge of being surrounded by biological macromolecules such as proteins upon entering the human body. These molecules compete for binding sites on the nanoparticle surfaces, forming a protein corona. The impact of different types of protein coronas on LNP delivery remains unclear. In this study, we employed a newly developed, highly sensitive LNP labeling platform and analyzed the endocytosis of HeLa cells under different nutritional conditions using proteomics to address this critical issue. Our research found that under conditions of complete medium and amino acid starvation, most DNA-FITC vesicles in HeLa cells were located in the perinuclear region 4 h after transfection. In contrast, under ser

SUBMITTER: Wang R 

PROVIDER: S-EPMC11510507 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature