Establishment of a Genetic Transformation and Gene Editing Method by Floral Dipping in <i>Descurainia sophia</i>.

ABSTRACT: Descurainia sophia L. Webb ex Prantl is used in traditional medicine globally. However, the lack of an efficient and reliable genetic transformation system has seriously limited the investigation of gene function and further utilization of D. sophia. In this study, a highly efficient, time-saving, and cost-effective Agrobacterium tumefaciens-mediated genetic transformation system has been developed in D. sophia. In this method, the transformation was accomplished by simply dipping developing D. sophia inflorescences for 45 s into an Agrobacterium suspension (OD₆₀₀ = 0.6) containing 5% sucrose and 0.03% (v/v) Silwet L-77. Treated plants were allowed to set seeds which were then plated on a selective medium with hygromycin B

SUBMITTER: Jia T 

PROVIDER: S-EPMC11510603 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature