An Antagonist Antibody That Inhibits Cancer Cell Growth In Vitro through RACK1.

ABSTRACT:

Background/objectives

Our research introduces a novel screening method to identify antibodies that can suppress cell proliferation and induce apoptosis.

Methods

By using an autocrine signaling system with lentivirus, we developed an antibody screening method based on FACS sorting assays and cell cycle analysis to inhibit tumor growth in vitro. This approach is particularly well suited for studying tumor suppressors. Inducing the G0 phase in tumor cells with specific antibodies may arrest their growth permanently or trigger apoptosis. The cell cycle is composed of tightly regulated phases for cell growth and division, with tumorigenesis or apoptosis occurring when these regulatory mechanisms fail.

Results

In our study, we identified RACK1 as a key regulator of cancer c

SUBMITTER: Kim JH 

PROVIDER: S-EPMC11510629 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature