BONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in <i>Toxoplasma gondii</i> to Pyrimethamine Treatment.

ABSTRACT: We employed a BONCAT-iTRAQ labelling approach to investigate newly synthesised proteins (NSPs) in Toxoplasma gondii subjected to varying concentrations of the antifolate drug pyrimethamine. Our results reveal that numerous NSPs exhibited altered expression levels in response to the drug, with significant upregulation observed at higher concentrations. Key proteins involved in protein synthesis, stress responses, energy metabolism, and cytoskeletal dynamics were identified, indicating that T. gondii undergoes complex adaptive responses to pyrimethamine treatment. While some of the identified pathways reflect a generic stress response, this study provides important molecular markers and mechanistic insights specific to the parasite's adaptation strategies. These findings contri

SUBMITTER: Mina JG 

PROVIDER: S-EPMC11510713 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature