The Recombinase Polymerase Amplification Test for <i>Strongyloides stercoralis</i> Is More Sensitive than Microscopy and Real-Time PCR in High-Risk Communities of Cusco, Peru.

ABSTRACT: Strongyloidiasis is a neglected, soil-transmitted helminth infection prevalent worldwide. The true burden of strongyloidiasis is unclear due to the lack of sensitive, field-friendly diagnostic tests. PCR tests to detect Strongyloides DNA in stool are sensitive and specific, but the need for expensive equipment limits their use in endemic regions. Isothermal PCR amplification tests are easier to deploy while maintaining sensitivity and specificity. We developed and evaluated a recombinase polymerase amplification lateral flow assay (RPA-LFA) to detect Strongyloides stercoralis in human stool samples. Three hundred stool samples were collected in three communities in the jungle of Cusco, Peru. Samples were tested for S. stercoralis larvae using microscopy (Baermann's, ag

SUBMITTER: Malaga JL 

PROVIDER: S-EPMC11510723 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature