Toxicity and Efficacy Evaluation of Soluble Recombinant Ricin Vaccine.

ABSTRACT:

Background

Ricin, a toxin extracted from the seeds of Ricinus communis, is classified as a ribosome-inactivating protein. The A-subunit of ricin shows RNA N-glycosidase activity that cleaves ribosomal RNA (rRNA) and exhibits toxicity by inhibiting protein synthesis and inducing vascular leak syndrome.

Methods

In this study, we created a truncated version of the previously developed R51 ricin vaccine (RTA 1-194 D75C Y80C) through in silico analysis.

Results

The resulting R51-3 vaccine showed a more-than-six-fold increase in soluble protein expression when compared to R51, with over 85% solubility. In a pilot toxicity test, no toxicity was observed in hematological and biochemical parameters in BALB/c mice and New Zealand white rabbits following five repea

SUBMITTER: Yun H 

PROVIDER: S-EPMC11511097 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature