An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration.

ABSTRACT: The anterior visceral endoderm (AVE) differs from the surrounding visceral endoderm (VE) in its migratory behavior and ability to restrict primitive streak formation to the opposite side of the mouse embryo. To characterize the molecular bases for the unique properties of the AVE, we combined single-cell RNA sequencing of the VE prior to and during AVE migration with phosphoproteomics, high-resolution live-imaging, and short-term lineage labeling and intervention. This identified the transient nature of the AVE with attenuation of "anteriorizing" gene expression as cells migrate and the emergence of heterogeneities in transcriptional states relative to the AVE's position. Using cell communication analysis, we identified the requirement of semaphorin signaling for normal AVE migration. Latt

SUBMITTER: Thowfeequ S 

PROVIDER: S-EPMC11511681 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature