Assessment of Nine Real-Time PCR Kits for African Swine Fever Virus Approved in Republic of Korea.

ABSTRACT: The African swine fever virus (ASFV) causes severe disease in wild and domestic pigs, with high mortality rates, extensive spread, and significant economic losses globally. Despite ongoing efforts, an effective vaccine remains elusive. Therefore, effective diagnostic methods are needed to rapidly detect and prevent the further spread of ASF. This study assessed nine commercial kits based on real-time polymerase chain reaction (PCR) approved in the Republic of Korea using the synthesized ASFV plasmid, 20 food waste samples, and artificially spiked samples (ASSs). The kits were evaluated for their diagnostic sensitivity, specificity, cost per reaction, and reaction running time. In addition, the results were compared with those of the World Organization for Animal Health (WOAH) standard meth

SUBMITTER: Lee S 

PROVIDER: S-EPMC11512253 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature