Long Terminal Repeats of Gammaretroviruses Retain Stable Expression after Integration Retargeting.

ABSTRACT: Retroviruses integrate into the genomes of infected host cells to form proviruses, a genetic platform for stable viral gene expression. Epigenetic silencing can, however, hamper proviral transcriptional activity. As gammaretroviruses (γRVs) preferentially integrate into active promoter and enhancer sites, the high transcriptional activity of γRVs can be attributed to this integration preference. In addition, long terminal repeats (LTRs) of some γRVs were shown to act as potent promoters by themselves. Here, we investigate the capacity of different γRV LTRs to drive stable expression within a non-preferred epigenomic environment in the context of diverse retroviral vectors. We demonstrate that different γRV LTRs are either rapidly silenced or remain active for long periods of time with a pr

SUBMITTER: Miklik D 

PROVIDER: S-EPMC11512309 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature