MiR-181a Negatively Regulates Claudin-3 to Facilitate <i>Lateolabrax maculatus</i> Iridovirus Replication in <i>Lateolabrax maculatus</i> Astroglia Cells.

ABSTRACT: Lateolabrax maculatus iridovirus (LMIV) is a variant strain of red sea bream iridovirus (RSIV), causing serious economic losses in aquaculture. Claudins (CLDNs) are major components of tight junctions (TJs) forming an important line of defense against pathogens. Our pilot miRNA-mRNA joint analysis indicated the degradation of CLDN3, as well as its interaction with miR-181a during LMIV infection. To elucidate the miR-181a/CLDN3/LMIV interactions, in vitro assays were carried out on LMB-L cells. We first confirmed that LMIV infection could decrease the expression of CLDN3, accompanied by the enhancement of permeability, suggesting the dysfunction of TJs. Contrary to the inhibition of CLDN3, the activation of miR-181a was proved, presenting a negative correlation between miR-181a and C

SUBMITTER: Ma Y 

PROVIDER: S-EPMC11512373 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature