Picornavirus Evolution: Genomes Encoding Multiple 2A^NPGP Sequences-Biomedical and Biotechnological Utility.

ABSTRACT: Alignment of picornavirus proteinase/polymerase sequences reveals this family evolved into five 'supergroups'. Interestingly, the nature of the 2A region of the picornavirus polyprotein is highly correlated with this phylogeny. Viruses within supergroup 4, the Paavivirinae, have complex 2A regions with many viruses encoding multiple 2A^NPGP sequences. In vitro transcription/translation analyses of a synthetic polyprotein comprising green fluorescent protein (GFP) linked to β-glucuronidase (GUS) via individual 2A^NPGPs showed two main phenotypes: highly active 2A^NPGP sequences-similar to foot-and-mouth disease virus 2A^NPGP-and, surprisingly, a novel phenotype of some 2A^NPGP sequences which apparently terminate translation at the C-

SUBMITTER: Luke GA 

PROVIDER: S-EPMC11512398 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature