Characterization of HIV variants from paired Cerebrospinal fluid and Plasma samples in primary microglia and CD4⁺ T-cells.

ABSTRACT: Despite antiretroviral therapy (ART), HIV persistence in the central nervous system (CNS) continues to cause a range of cognitive impairments in people living with HIV (PLWH). Upon disease progression, transmigrating CCR5-using T-cell tropic viruses are hypothesized to evolve into macrophage-tropic viruses in the CNS that can efficiently infect low CD4-expressing cells, such as microglia. We examined HIV-1 RNA concentration, co-receptor usage, and CSF compartmentalization in paired CSF and blood samples from 19 adults not on treatment. Full-length envelope CSF- and plasma-derived reporter viruses were generated from 3 subjects and phenotypically characterized in human primary CD4⁺ T-cells and primary microglia. Median HIV RNA levels were higher in plasma than in CSF (5.01 vs. 4.

SUBMITTER: Gumbs SBH 

PROVIDER: S-EPMC11512886 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature