A highly sensitive and specific real-time quantitative polymerase chain reaction assay for Perkinsus marinus detection in oysters.

ABSTRACT: Oyster aquaculture is one of the fastest-growing food production industries worldwide; however, it faces a significant challenge from the protist Perkinsus marinus, particularly in the USA. Although several quantitative molecular diagnostic methodologies are available for identifying diseases caused by P. marinus, the primer pairs used therein led to non-specific identification of other Perkinsus spp. Hence, a quantitative real-time PCR (Pm-qPCR) assay specific for P. marinus was developed using a TaqMan-based probe with the internal quencher in this study. A primer pair and probe specific to P. marinus were designed from a hypothetical protein of P. marinus collected from the whole-genome shotgun sequence database of the National Center for Biotechnology Information (NCBI). In silico anal

SUBMITTER: Kim SH 

PROVIDER: S-EPMC11513098 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature