MiR-10a regulates cell death and inflammation in adipose tissue of male mice with diet-induced obesity.

ABSTRACT:

Objective

Adipose tissue remodeling plays a critical role in obesity-induced metabolic dysfunction, but the underlying molecular mechanisms remain incompletely understood. This study investigates the role of miR-10a-5p in adipose tissue inflammation and metabolic dysfunction induced by a high-fat diet (HFD).

Methods

Male miR-10a knockout (KO) mice were fed a HFD to induce obesity for up to 16 weeks. RNA sequencing (RNA-seq) analysis was performed to profile mRNA expression and assess the effects of miR-10a-5p KO in gonadal white adipose tissue (gWAT). Additional analyses included immunoblotting, qPCR, histological examination, and validation of the miR-10a-5p target sequence using a dual-luciferase reporter assay.

Results

miR-10a-5p was highly expressed in gWAT but de

SUBMITTER: Lee S 

PROVIDER: S-EPMC11513492 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature