Ligand presentation controls collective MSC response to matrix stress relaxation in hybrid PEG-HA hydrogels.

ABSTRACT: Cell interactions with the extracellular matrix (ECM) influence intracellular signaling pathways related to proliferation, differentiation, and secretion, amongst other functions. Herein, bone-marrow derived mesenchymal stromal cells (MSCs) are encapsulated in a hydrazone crosslinked hyaluronic acid (HA) hydrogel, and the extent of stress relaxation is controlled by systemic introduction of irreversible triazole crosslinks. MSCs form elongated multicellular structures within hydrogels containing RGD peptide and formulated with elastic composition slightly higher than the hydrogel percolation threshold (12 % triazole, 88 % hydrazone). A scaling analysis is presented ( < R g Structure 2 > 1 2 ∼N^α) to quantify cell-material interactions within these structures with the scali

SUBMITTER: Borelli AN 

PROVIDER: S-EPMC11513683 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature